mass spectrometry-based proteomics in the life sciences: a review
نویسندگان
چکیده
proteomics concerns itself with the characterization and function of all cellular proteins, the ultimate determinants of cellular function. mass spectrometry has emerged as the preferred method for in-depth characterization of the protein components of biological systems. using mass spectrometry, key insights into the composition, regulation and function of molecular complexes and pathways have been gained. now days, mass spectrometry-based proteomics has become an indispensable tool in the cellular and molecular life sciences. this review discusses current mass spectrometry-based proteomics technologies.
منابع مشابه
Computational Mass Spectrometry–Based Proteomics
Proteomics is defined as the system-wide characterization of all the proteins in an organism in terms of their sequence, localization, abundance, post-translational modifications, and biomolecular interactions. Modern proteomic investigations are increasingly quantitative and comprehensive [1]. Examples include the relative quantification of over 4,000 proteins in haploid and diploid yeast, whi...
متن کاملMass spectrometry–based proteomics in cell biology
The global analysis of protein composition, modifications, and dynamics are important goals in cell biology. Mass spectrometry (MS)-based proteomics has matured into an attractive technology for this purpose. Particularly, high resolution MS methods have been extremely successful for quantitative analysis of cellular and organellar proteomes. Rapid advances in all areas of the proteomic workflo...
متن کاملQuantitation in mass-spectrometry-based proteomics.
Mass-spectrometry-based proteomics, the large-scale analysis of proteins by mass spectrometry, has emerged as a new technology over the last decade and become routine in many plant biology laboratories. While early work consisted merely of listing proteins identified in a given organ or under different conditions of interest, there is a growing need to apply comparative and quantitative proteom...
متن کاملMass spectrometry in proteomics.
4. Automated Interpretation of CID Spectra 282 5. Accurate Mass Tags 282 C. Protein Identification in Complex Mixtures 282 D. Analysis of Protein Expression 284 III. Proteomes and Post-Translational Modifications 285 A. Proteomes 285 1. The Analytical Challenge 285 2. Analysis of Protein−Protein Complexes 286 B. Post-Translational Modifications 286 1. Background 286 2. Detection and Purificatio...
متن کاملQuantitative mass spectrometry in proteomics: a critical review.
The quantification of differences between two or more physiological states of a biological system is among the most important but also most challenging technical tasks in proteomics. In addition to the classical methods of differential protein gel or blot staining by dyes and fluorophores, mass-spectrometry-based quantification methods have gained increasing popularity over the past five years....
متن کاملQuantitative mass spectrometry-based proteomics: an overview.
In recent years, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review the strategies and problems of the methods current...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of paramedical sciencesجلد ۱، شماره ۱، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023